Tricine ?Biological Buffer ?Tris(hydroxymethyl)methylglycine CAS NO 5704-04-1

Quick detail:

Description:

>?Formula:C6H13NO5

>?Weight:?179.17

>?MP/BP :180-186℃

> Storage:room?temperature

>?Safety?Description:S26 S36/37/39

>?Danger Description:R36/37/38

>?Danger Symbol:?Xi

Synonyms:

LABOTEST-BB LT00454966;n-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]-glycin;TRICINE;TRI(HYDROXYMETHYL) AMINO METHANE HYDROCHLORIDE;TRIMETHYLOLAMINOMETHANE HYDROCHLORIDE;TRIS-HCL;TRIS-HCL BUFFER;TRIS HYDROCHLORIDE;N-(2-Hydroxy-1,1-bis(hydroxymethyl)ethyl)glycine; N-[Tris(hydroxymethyl)methyl]glycine; Tricine N-Tris(hydroxymethyl)methylglycine; N-(Trishydroxymethylmethyl)glycine; N-(tri(hydroxymethyl)methyl)glycine; Tricine, Molecular Biology Grade N-Tris(hydroxymethyl)methylglycine, Molecular Biology Grade; Tricine, ULTROL Grade; N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine; LABOTEST-BB LT00454966; n-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]-glycin; TRI(HYDROXYMETHYL) AMINO METHANE HYDROCHLORIDE; TRIMETHYLOLAMINOMETHANE HYDROCHLORIDE; TRIS-HCL BUFFER; N-[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]glycine

Related categories:Biochemistry;Good\'s Buffers;Buffer

Related Products:60-32-2

The?determination?of?PH

Weigh?a?certain?amount?of?samples?into?test?tube,?add?a?certain?amount?of?water,?shake?and?dissolve,?determination?of?the?pH?used?have?been?calibrated?pH?meter.

The?determination?of?solubility

Weigh?a?certain?amount?of?samples?into?test?tube,?add?a?certain?amount?of?water,?shake?and?dissolve,?observe?the?solution.

The?determination?of?KF

Use?MA-1?intelligent?Carle?Fischer?moisture?tester?to?measure?water?content

The?determination?of?UV?absorbance

??A?certain?amount?of?the?sample?was?weighed?to?the?nearest?0.0001g,?into?10ml?volumetric?flask,?after?constant?volume?with?pure?water,?at?a?specific?wavelength?by?2cm?cuvette?was?measured?by?UV?spectrophotometer.

Detection?of?heavy?metals

?????The?meaning?of?heavy?metals?of?this?act?is?under?the?specified?experimental?condition,?which?can?coloration?effects?with?sodium?sulfide?or?thioacetamide??.The?glasses?of?preparation?or?storage?is?not?allowed?to?contain?lead.

5.8.1??Reagents

??a)?Lead?dinitrate:1μg/mL

???b)?Sodium?hydroxide?:10%(m/v)

???c)?Sodium?sulfide?:10%(m/v)

5.8.2??Preparation?of?lead?standard?solution

?????Weigh?lead?dinitrate??0.1599g?,relocated?to?1000mL?volumetric?flask,?plus?nitric?acid?5mL?and?water?50mL,?diluted?with?water?to?the?mark?,?shake,?as?the?stock?solution.

5.8.3?Determination?of?step

???Before?use,?the?exact?amount?of?stock?solution?1mL,?relocated?to?a?100ml?volumetric?flask,?dilute?with?water?to?the?mark,?shake,?limit?the?use?of?the?day?.

???Weigh?sample?1g,?after?adding?sodium?hydroxide?solution?5mL?and?20mL?of?water?to?dissolve,?set?Nessler?colorimetric?tube,?add?5?drops?of?sodium?sulfide?,?shake?,?compared?the?color?with?5mL?lead?standard?solution?after?the?same?treatment,??not?deeper.

Determination?of?melting?point

???Instrument:

???Micro?melting?point?apparatus?WRS-2A,?Melting?point?tubes

??Determination?of?step:

Open?switch,?stable?20min.

First?set?the?starting?temperature?than?the?melting?point?of?the?low?10?℃,?heating?rate?2?℃?/?min.

The?sample?was?grind?as?fine?as?possible?and?load?in?a?clean,?dry?melting?point?pipe?,until?the?sample?was?compressed?into?about?1cm?high?.When?the?actual?temperature?reaches?the?set?temperature?and?stabilized?.?Inserted?into?the?capillary?with?the?sample,?the?simultaneous?determination?of?three.