Hesperidin has extensive bacteriostatic effect on common contaminating bacteria in food, and has significant inhibitory effect on Bacillus subtilis, Salmonella typhimurium, Shigella refrigerators, Streptococcus hemolyticus and Vibrio cholerae, so it is widely used in food additives and food processing.

Hesperidin has the functions of maintaining osmotic pressure, enhancing capillary toughness, shortening bleeding time and lowering cholesterol. It is used for clinical auxiliary treatment of cardiovascular diseases, and can develop a variety of drugs to prevent arteriosclerosis and myocardial infarction. It is one of the main raw materials of Chinese patent medicine "vascular patency".