99% CAS NO 150-25-4 Biological Buffers BICINE ?N,N-Bis 2-Hydroxyethyl Glycine ?

Quick Detail:

Description:

>?Formula:?C6H13NO4

>?Weight:?163.1717

>?MP/BP:190-192℃(MP)

> Storage:?room?temperature

>?HS:2922499990

>?Safety?Description:?S24/25

Applications:Biological buffer

Synonyms:

DI(HYDROXYETHYL)GLYCINE;DIETHYLOLGLYCINE;DIETHANOLGLYCINE;[BIS-(2-HYDROXY-ETHYL)-AMINO]-ACETIC ACID;BICINE;BICINE BUFFER;LABOTEST-BB LT00452695;n,n-bis(2-hydroxyethyl)-glycin;Diethylolglycine; N,N-Bis(2-hydroxyethyl)glycine; BICINE = N,N-Bis(2-hydroxyethyl)glycine; Bicine, Molecular Biology Grade N,N-Bis(2-hydroxyethyl)glycine, Molecular Biology Grade; Bicine, ULTROL Grade; BICINE buffer Solution; BICINE, [N,N-Bis(2-hydroxyethyl)glycine]; N,N-Di(2-hydroxyethyl)glycine; N,N-Di(2-hydroxyethyl)-glycine; N,N-dihydroxyethylglycine

Related categories:Biochemistry;Good\'s Buffers;Buffer

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Determination?of?melting?point

???Instrument:

???Micro?melting?point?apparatus?WRS-2A,?Melting?point?tubes

??Determination?of?step:

Open?switch,?stable?20min.

First?set?the?starting?temperature?than?the?melting?point?of?the?low?10?℃,?heating?rate?2?℃?/?min.

The?sample?was?grind?as?fine?as?possible?and?load?in?a?clean,?dry?melting?point?pipe?,until?the?sample?was?compressed?into?about?1cm?high?.When?the?actual?temperature?reaches?the?set?temperature?and?stabilized?.?Inserted?into?the?capillary?with?the?sample,?the?simultaneous?determination?of?three.

The?determination?of?PH

Weigh?a?certain?amount?of?samples?into?test?tube,?add?a?certain?amount?of?water,?shake?and?dissolve,?determination?of?the?pH?used?have?been?calibrated?pH?meter.

The?determination?of?UV?absorbance

??A?certain?amount?of?the?sample?was?weighed?to?the?nearest?0.0001g,?into?10ml?volumetric?flask,?after?constant?volume?with?pure?water,?at?a?specific?wavelength?by?2cm?cuvette?was?measured?by?UV?spectrophotometer.

The?determination?of?Loss?on?drying

??Weigh?a?sample?of?approximately?2g?(weighing?accuracy?0.0001g),Record?the?weight?of?m,The?sample?was?placed?in?a?Petri?dish?constant?weight?(weight?recorded?as?m0),?the?tile,?the?thickness?of?not?more?than?5mm,?into?the?oven?(As?next?dish?cover?removed)?drying?at?105-110?℃?3?hours.When?removing,?make?sure?the?dish?cover,be?placed?in?a?desiccator?to?cool,?then?weighed?and?the?weight?(including?surface?dish)?m1.

Loss?on?drying?in?accordance?with?the?following?formula:

Where:

?????????m--?sample,?g

???????m0--?dish,?g

??????????m1--?After?drying?the?quality?of?the?sample?and?the?dish,?g